what is method development parameter in HPLC method
development
Answers were Sorted based on User's Feedback
Answer / abdulkalim
Parameters involved during initial stage of Analytical
Method development:
1. Optimization of Mobile Phase
2. Selection of Buffers
3. Selection of Ion pair reagent
4. pH of buffer/mobile phase
5. Composition of Mobile Phase
6. Selection of Column
7. Optimization of Analyte signal
8. Objectives of Seperation
8.1 Analysis time
8.2 Resolution
8.3 k factor (Capacity factor)
8.4 Peak height
8.5 Asyymetry
8.6 Theoritical Plates
9. Flow rate
Is This Answer Correct ? | 90 Yes | 7 No |
Answer / ramana
Parameters involved during initial stage of Analytical
Method development:
1. Solubility
2. Pka Value
3. Selection of Buffers
4. pH of buffer/mobile phase
5. Composition of Mobile Phase
6. Selection of Column
7. Optimization of Analyte signal
8. Objectives of Seperation
8.1 Analysis time
8.2 Resolution
8.3 k factor (Capacity factor)
8.4 Peak height
8.5 Asyymetry
8.6 Theoritical Plates
9. Flow rate
Is This Answer Correct ? | 54 Yes | 2 No |
Answer / yojana
1. Check solubility,if sample dissolved in water then use
reverse phase,othervise use normal phase.
2.wavelength selection
3.selection of mobile phase,buffer.
4. pH OF BUFFER &MOBILE PHASE
5. selection of column
6. check retention time
7 .Flow rate
8. check system suitability parameter like
i)theoritical plates
ii) Assymetry
iii) Resolution
iv) capacitor fator
Is This Answer Correct ? | 31 Yes | 2 No |
Answer / vinod kumar, nektar therapeuti
1.If molecular weight less than 2000. based on their
solubility follow as below
2.If water soluble and non ionic follow reverse phase.
3.If water soluble and ionic follow reverse phase ion pair
method.
4.If organic soluble follow normal phase.
5.If molecular weight is more than 2000.Based on their
solubility follow as below.
6.If organic soluble follow gel permeation chromatography
7.If water soluble ion exchange or gel filtration.
8.Once the technique is fixed optimise column.
9.Optimise buffers
10.optimise pH of buffers
11.optimise concentration of buffers.
12.optimise type of organic solvent.
13.optimise column temperature
14.optimise gradient programme to improve resolution and to
elute out late eluting peaks.
Is This Answer Correct ? | 19 Yes | 11 No |
Answer / sivannarayana
1.Solubility
2.Find out the UV scan For wave length
3.optimaize Buffer & pH Value Optimaize
4.Optimaize Column Temparature & optimaize sample cooler Temparature.
5.optimaize the GP For better Resolution
Is This Answer Correct ? | 4 Yes | 0 No |
Answer / anand patel
as per my knowledge system suitability test is the method
delopment parameter
Is This Answer Correct ? | 10 Yes | 19 No |
Please do not join if u got offer in oncotherapies ltd (STRIDESARCOLAB)especially Mr.Mahesh Bhatt team because job is not secure his team and doesn't have confidence on him becareful this offer?
If compound Pka is 5 then which buffer you will select ?
Why do we need GC instead of HPLC? or Why do we need HPLC instead of GC?
Why do we use 0.005M h2so4 solution for preparation of k2cr2o7 solution for UV calibration?
3 Answers Jubilant, Medreich, Mylan,
why use k2cr2o7 , kcl h2so4 in uv calibration
If mass balance is not passing in forced degradation in method validation that method is suitable or not?
1 Answers Aurobindo, Dr Reddys, Reddy Labs, Wockhardt,
if content uniformity passing but dissolution varrying then what is next step?
why reference flow required during hplc analysis on rid?
what is the diffrence between absorbance and absorption in the beer,s lamber formula ?
if peak get problamatic then what require to do?
wt is rsd in and explain
What is the difference between Normal phase method & Reverse method in HPLC ? Define application of both method.