Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography?
Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18
what is the concept for adjustment of pH in HPLC buffer solution ?
a. Common ion effect
b. Counter ion effect
and explain me, how we adapt this principles & what is the role of these principles in our method