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what is method development parameter in HPLC method
development
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Answer Posted / sivannarayana
1.Solubility
2.Find out the UV scan For wave length
3.optimaize Buffer & pH Value Optimaize
4.Optimaize Column Temparature & optimaize sample cooler Temparature.
5.optimaize the GP For better Resolution
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My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards
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