What is the difference between void volume and delay volume
in HPLC?
Answers were Sorted based on User's Feedback
Answer / kishor solanke(alkem)
Delay Volume is the volume between GPV & Detector.
Dead volumn is the volume between sample injector & Column
outlet.
Is This Answer Correct ? | 26 Yes | 5 No |
Answer / rajendra baraskar
Delay Volume is the volume between GPV & Detector.
Dead volumn is the volume between sample injector & Column
outlet.
Is This Answer Correct ? | 5 Yes | 0 No |
Answer / priyanka nayani
Delay volume is the volume between GPV & Detector.
Dead volume is the width of pipe that is the connection between column out let and detector(0.1mm or 0.01mm) based on the size which we have introduced the void volume changes.
Is This Answer Correct ? | 0 Yes | 1 No |
In Karl Fischer titration,What is the situation if the material tested is only soluble in water,and not soluble in the common solvents used such as methanol ?? [e.g. Iron III Hydroxide polymaltose]
What is the column performance and column efficiency?
how decide the clining method and cleaning method validation require for this perticular products?
identification is for unknown? qualification for known? reporting for LOQ?
Which are the diffrent grades of api in pharma?
how can standardized of HCl of 0.1 N
what is main use of primary standards in caliration
2 Answers Lee Pharma, Lupin, Nectar Lifesciences,
why we are using only lead standard solution for Heavy metals test? what is the purpose of this?
What is AV value in test uniformity content ,describe calculation
How to Calibrate NMR instrument ?
how to selecet an exact coloumn for an new molecule development by hplc how to select exact salt as buffer for new molecule development by hplc what is the the process to select the mode of saparation of compoundes by hplc what is the use of ph of buffer what is use of buffer,ph,organic phase,ans methods how the molecules get saparated in coloumn,
in hplc calibration we are using 0.05% acetone why we are using that one and what happen when its conc increases or decreases
4 Answers Aurobindo, Cadila, Martin,