Interested to Buy Any Domain ? << Click Here >> for more details...
Dear Sir,
This is Thirunavukkarasu am working in Ordain Health Care
AR&D, Am Having one problem While doing method development
my question Metadoxine and Pyridoxine same chemical nature
and structural activity i tried lot of methods but Analyte
eluted same Retantion time i cant separate both peaks.
solubility, pH both are same only only difference melting
point metadoxine 210 C Pyridoxine 102 C please do the help
needful for me
- 5 Answers
- 9755 Views
- Ordain Solutions, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / bpatel1773
Dear ,
I thing you want to isolate both compound , You can try
derivatization method using bezoyl chloride or densyl
chloride . Bezoyl chloride will react with only metadoxine
and it will eluted late on HPLC . This way must work .
Benzoy chloride will react at RT only
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / a.prabhakar
Dear Thirunavukkarasu,
What is your column flow rate and have you change column flow rate.
| Is This Answer Correct ? | 2 Yes | 1 No |
Answer / manoj kumar vadlamudi, ashla
Dear SIR,
Kindly play with different pH also...
and also try with RID detector....
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / swathi
Once u can use hilic silica column ..mostly water %dont exceed 20% in gradient program otherwise phenyl column particle size 3micron..slow gradient ...flow rate decrease..and at which they are eluting there keep isocratic for 30 min
Is it the compounds are different?
| Is This Answer Correct ? | 0 Yes | 0 No |
what is the purpose of Buffer in any titration?Ex:In determination of hardness of water we use ammonia ammoniachloride buffer in titration with EDTA.Why?
13 Answers Amity, Prakriti, Sr Comunications,
what is the difference between melting range & melting point? Which is more significant?
why peak purity not considered in GC?
4 Answers DRL, Reddy Labs, Vimta Labs,
Why the same type of columns showing different RT for a single product?
What is method validation in quality control
What is importance of pH in the Heavy metal test and How it effect on the test results?
what is the meaning of ventillation in HPLC?
3 Answers Amneal Pharmaceuticals,
What is the pilot-plant in pharma sector.
what is the difference between EP and BP
2 Answers Novartis, Vimta Labs,
What are usp limits for total impurities,unknown impurity
what is the meaning of stress study?
Basic principle of ESI?
Organic Chemistry 
