How do I grow hybridoma cell lines as ascites, especially
rat-mouse hybridomas?
Answer / guest
ATCC propagates most hybridomas as cell cultures in vitro,
but many can be grown efficiently as ascites by inoculation
into the peritoneal cavities of mice. Some rat-mouse
hybridomas are more problematical as the host mouse strain
may develop anti-rat antibodies and eventually reject the
hybridoma inoculum. To minimize this difficulty one can use
athymic nude mice as hosts or immunosuppress host animals
prior to inoculation
Is This Answer Correct ? | 0 Yes | 1 No |
What do facilitated diffusion and active transport have in common? What are the differences between them?
Which are the cell organelles that participate in the cell division and in the formation of cillia and flagella of some eukaryotic cells?
Describe about 3T3 and serum ?
What are plant cell vacuoles? What are their functions?
What is lncap cell culture ?
What is cell biology?
what is Cellular Signal Transduction ?
What happens inside of lymph nodes?
How many cells (nuclei) per ml pellet?
Which amount of DMEM and Glutamine should be commaneded ?
How to Measure nitrite from LPS-activated RAW264.7 cells ?
What do you know about the Transfection of CHO-K1 cells?