How do I grow hybridoma cell lines as ascites, especially
rat-mouse hybridomas?
Answer Posted / guest
ATCC propagates most hybridomas as cell cultures in vitro,
but many can be grown efficiently as ascites by inoculation
into the peritoneal cavities of mice. Some rat-mouse
hybridomas are more problematical as the host mouse strain
may develop anti-rat antibodies and eventually reject the
hybridoma inoculum. To minimize this difficulty one can use
athymic nude mice as hosts or immunosuppress host animals
prior to inoculation
Is This Answer Correct ? | 0 Yes | 1 No |
Post New Answer View All Answers
will the passage number affect the performance of THP-1 cells?
How the chromosome region where the centromere is located is called? How are chromosomes classified in relation to the position of their centromere?
Does the ATCC do MAP (mouse antibody production) testing?
What is the function of the plant cell wall?
What are plant cell vacuoles? What are their functions?
Describe about Fusing spleen cells with myeloma cells using PEG ?
what is the transfection reagent for HT-29 and HCT-116 cells ?
Describe the Monocytic cell lines ?
Where in the cell can ribosomes be found? What is the main biological function of ribosomes?
What are the main cytoplasmic structures present in animal cells?
How to detect apoptosis?
Regarding FBS - What is the difference between the grades?
how to detect and de-contaminate, Mycoplasma contamination?
What are some examples of ciliated and flagellated cells in humans?
Are there ribosomes in a plant cell?