Answer / vineel

Xylitol is a five-carbon sugar alcohol that is used as a
sugar substitute. Xylitol is a naturally occurring sweetener
found in the fibers of many fruits and vegetables, including
various berries, corn husks, oats, and mushrooms. It can be
extracted from corn fiber, birch, raspberries, plums, and
corn. Xylitol is roughly as sweet as sucrose but contains
40% less calories.

I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

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In sickle cell anemia, a hereditary disease, there is substitution of one amino acid by other in one of the four-polypeptide chains of hemoglobin. In this case, are all of the structural levels of the protein modified?

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explain about hyperglycemic agent?

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In water molecule the bond angle decreases from 109.28 to 104.5, why this does happens?

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Glycolysis is A) C6H12O6 --> 2C2H5OH + 2CO2 B) C6H12O6 --> 2CH3H4O3 + 4H c) C3H4O3 + NAD --> C2H5OH + CO2 NAD D) C6H12O6 + 6H20 --> 6CO2 + 6H2O

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What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?

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What are the salient features of chemiosmotic coupling hypothesis?

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Is there any metabolic pathway for water? If not is it possible to provide one?

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what is ramachandran plot?what r its applications?

18 Answers   Biocon, GVK, SAD,

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is there a hormone in our body which when released induces sleep in us?is there a spurt in the secretion of that hormone in adolescence?why?

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if hydrogen ion concentration of a solution is 90% what will be the ph of that solution?

2 Answers   BITS,

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What are the common features of active site?

1 Answers  

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