MICROBIOLOGICAL ENGINEERING - QUESTION 28.2 : A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells occupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).

1 Answers  

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white rust formation over zinc plated and yellow passivated fasteners during CED painting. WHY?

0 Answers   Wheels India,

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What is difference between a Flue Gas Analyzer and Exhaust Gas Analyzer ?

1 Answers  

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What is the wet bulb globe temperature (wbgt)?

0 Answers  

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what is density

2 Answers   CPCL, TII,

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when selecting psv(pressure safety valve),what are all things we have to keep it in mind?

4 Answers   Qasco,

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Repected sir/Madam, I am goingb to face IOCL technical as well as HR interview, can you forward your hellpfull hand for toward me to sort out my problem by giving some imp interview quetion which is generally asked in chemical industries such like IOCL,HPCL,ONGC,BPCL,RIL,etc I will always thankfull to you,for sharing your profficient knowledge with me and making me somewhat technically sound

0 Answers   IOCL,

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What is the ignititon temprecher of Diesel,Petrol&Carosion oil.

0 Answers   ME,

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how much maximum power can be generated by 320v, 10kg-cm synchronus motor if shaft is roteted mechanically at 50 to 60 rpm?

0 Answers  

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Is there a difference in mtd (mean temperature difference) between 'e' and 'j' (divided flow) type shell and tube heat exchangers?

0 Answers  

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What are the types of clarifier used in water treatement plant?

1 Answers   Adani,

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BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.3 : In thin layer chromatography (TLC), let retention factor Rf = (distance traveled by solute) / (distance traveled by solvent). Silica gel is used as stationary phase which is more polar than the hexane solvent as mobile phase. If the Rf values of 3 solutes A, B and C are 1.5, 0.75 and 1 respectively, compare the polarities of A, B and C with reasons.

1 Answers  

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