Name two elements, which are non-metallic in nature in 5th a group?
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What is dsDNA said to be antiparellel?
What are respectively some remarkable functions of keratin?
what is the wood sugar?
how the keratosulphate differs from other mucopolysaccharides?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
What are the units of ph?
Why does ion-exchange chromatography generally use a salt gradient or a pH gradient?
Which theory explains the paramagnetic nature of oxygen? Who proposed it?
what are muco polysaccharides?
What is the structural formula of molybdenum blue?
what are eicosanoids?name the three subclasses of eicosanoids?
What is kararch effect?