How to check hplc detecter sensitivity?
Answer Posted / babasaheb gilbile
In Waters HPLC 1 ml flow for methanol passing with wavelenth 230nm. and after 1hr check the Detector lamda max value. If ie greater than 20 the detector sensitivity is good
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I have compare C2H2-air and C2H2-N2O flame AAS on determination calcium. Both use same range of std to plot calibration curve. (2-6ppm) When i measure the sample with phosphate, KCl and LaCl, C2H2-N2O flame give false positive result, around 0.5ppm. When i measure the sample with phosphste, KCl and EDTA. C2H2-N2O flame also give 0.5ppm false positive. But both above mentioned sample would not give false positive when measured by C2H2-air flame. What is the reason?
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