What is column in chromatography?


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In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.

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In HPLC Chromatographic separation Resolution equation is Rs = 1.18 (tR2 –tR1)/wh1+Wh2, Where, tR2 and tR1 is run time of eluent ratios of the distances from the point of application to the centers of the spots and the distance travelled by the solvent front from the point of application (retardation factor), and Wh1and Wh2 , is peak width of at half height. What is 1.18 ?

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How require to perform linearity as per ANVISA? What are the acceptance criteria?

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state the principle of gas chromatography?

5 Answers  


who is the father of HPLC?

6 Answers   Cipla, IPCA,






why in hplc peaks are in positive side(it means upside) not in negative side?

3 Answers  


how much mass should be there in volumetric flask while in preparation of sample for assay? 

0 Answers   Reddy Labs,


Why noise test is performed at 254 nm in calibration of HPLC system?

4 Answers  


How to know the estimated LOQ concentration in ppm

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principle of FTIR

2 Answers   Biocon, Macleods,


Which water can use Hplc analysis

9 Answers   BioGenomics, Dr Reddys, Reddy Labs,


What impact analysis required for out of calibration and how to proceed

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