How much 2-mercaptoethanol 0.5M should be added to medium for pbmc
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Answer / Vikas Kumar Rao
To determine the exact amount of 2-mercaptoethanol (0.5M) required for a specific volume or weight of PBMC (Peripheral Blood Mononuclear Cells), you would need to refer to the specific protocol or guidelines used in your laboratory. However, a common practice is to add 10% vol/vol 2-mercaptoethanol to the cell suspension medium. This means if you have 100ml of medium, you would add 10ml of 2-mercaptoethanol (0.5M).
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