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What are the differences between GC and HPLC?
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Answer / g. subrahmanyam
The difference between HPLC and GC are as follows.
a. In HPLC the mobile phase is a liquid whereas in Gas
Chromatography the mobile phase or carrier is a gas.
b. HPLC is useful for analysis of samples which are
liable to decompose at higher temperatures. GC involves high
temperatures, so compounds are stable at such temperatures.
c. Gas Chromatography is applied for analysis of
volatile compounds whereas non volatile compounds can be
easily analyzed on HPLC
d. Gas Chromatography cannot be used for analysis of
high molecular weight molecules whereas HPLC has
applications for separation and identification of very high
molecular weight compounds
e. HPLC requires higher operating pressures than GC
because liquids require higher pressures than gases for
transport through the system
f. HPLC columns are short and wide in comparison to GC
columns
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