Interested to Buy Any Domain ? << Click Here >> for more details...
What is the superiority of TLC over paper chromatography?
- 9 Answers
- 40754 Views
- Ranbaxy, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / ravi
ADVANTAGES OF TLC OVER PAPER
1. high speed
2. high sensitivity
3. small sample required
4. sharper preparation of sample
5. various reagents are used without damaging plates
6. large choice of adsorbents
7. after development , sample easily separated
| Is This Answer Correct ? | 79 Yes | 3 No |
Answer / kavitha jai
when compare to paper chrmatograpy TLC having more
advantage because in TLC we are having wide joice of
adsorbent depend upon our sample and development. after
development sample can seperate easily from TLC. higher
version of TLC like HPTLC and more type is their for
purification of the sample.
| Is This Answer Correct ? | 29 Yes | 8 No |
Answer / s.sindhu
the superioity of TLC over paper chromatography is TLC
requires less time for separation of aminoacids,plant
pigments etc
| Is This Answer Correct ? | 24 Yes | 5 No |
The superiority of TLC over paper chromatography
because of inorganic nature of adsorbent concentrated
sulfuric acid spray followed by heating may be used to
develope on the chromatogram by charring.
moreover aminoacid mixtures which require 18 hours for
seperation on paper require 3 hrs using cellulose TLC.
advantage also lies in choice of adsobents which allow
seperation not possible on paper.
| Is This Answer Correct ? | 21 Yes | 15 No |
Answer / victoria
In tlc the stationary phase is a silica gel plate vt a supprting medium wher asin papr the sstationary phase is d water present in cellulose. More crrosive reagents can b used in tlc eg. Sulfuric acid, wher as corrosive rgnts cn spoil d stationary phse in paper chromatography.. tlc is a faster less time consuming technique n seperation of d spots is also faster.. most importantly the proper sepearration of d spots in tlc is due to d particle size o f d stationary phase.. here the partcles are evenly packed n siz is smaller ..
| Is This Answer Correct ? | 4 Yes | 0 No |
Answer / swadhin ghosh
1) Higher sample throughput and lower analysis time, as the number of samples that can be separated on one plate in TLC, is far more than the number of samples that can be separated in one paper.
2)Strong reagents can be used for the purpose of identification, as the TLC plate is able to withstand strong solvents and colour-forming agents.
3)TLC plates can be heated to higher temperatures(for spot development) without any damage, which might not be possible in case of PC, lest the paper gets damaged.
4)The sensitivity of detection is more in TLC, as compared to PC, because of lesser spreading of substance zones, in a TLC plate, than what occurs in PC.
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / syeda humaira
TLC is superior over paper in most of the ways but the most
important one is,on TLC plate corrosive reagents can be
used for identification of spots but in paper it gets
charred and the sample spot gets destroyed
| Is This Answer Correct ? | 12 Yes | 11 No |
Answer / shafira
InTIC,silica gel acts as a stationary phase.
In PC, water present in the cell use of paper is stationary phase
| Is This Answer Correct ? | 2 Yes | 3 No |
Answer / gayatri
in tlc silica gel is used as ststionary phase. in paper
cromatography paper itself is used as a stationary phase
| Is This Answer Correct ? | 7 Yes | 10 No |
I've an interview for post of Lecturer in a Biotechnology Department. Can anyone send me some possible questions that can be helpful for me???????
What is meant by transversion?
Difference between water pollinated and wind pollinated
1 Answers St Pauls KG & Day School,
components of bioreactor
What are the problems we could face by using this genetic engineering tools?
Why do you want to do phd?
who is the father of biotechnology
Which form of plasmid DNA moves quickest thro Agarose gels?
Sir i completed m.sc.biotech but for good career i want to do some diploma courses for good job . Will you please suggest me the coures
I am an B.Tech Industrial biotechnology fresher(2010 passed out). I am looking for job in biotech companies. I approached some companies too. But in all companies they said they need experienced persons only and not freshers. Please suggest me a solution to my job search. And suggest me some biotech companies where fresher are hired.
what is catabolic repression?
Name the dye commonly used as the counterstain in the Gram staining process.
Micro Biology 
