Interested to Buy Any Domain ? << Click Here >> for more details...
What is use of LOD and LOQ in validtion and give range and in calculation why we use 3.3* LOD here why we use 3.3
- 2 Answers
- 6692 Views
- Dr Reddys, IPCA, Reddy Labs, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / nikkosthiru@gmail.corai12
LOD is Limit of detection. We can dedect the peak but cant quantify
LOQ Limit od quaantification. We quantify also this is known as reporting thereshold.
During the RS analysis Below LOQ level we cant included in the total impurities calculation.
LOD and LOQ Determination three methods
1.S/N Ratio method
3 or 2 ratio for LOD
NLT 10 ratio for LOQ
2. Slope Method
Plot the linearity for active and impurities through serial dilution at lower level
Formula for LOD
3.3*Slope/Std.dev
Formula for LOQ
10*Slope/Std.Dev
3. Visualization method
It will be using for only identification and TLC Method
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / kovida
LOD and LOQ are the smallest amount of the particular compound that can by detected and quantiified by using the developed HPLC method.
The signal to noise ratio is that minimum amount which when injected in HPLC it gives minimum detectable peak area.
The value of amount at this point is multiplied by 3 to get LOD
and by 10 to get LOQ value. In short LOD is the thrice value of minimum value and LOQ is ten times of minimum value that can be injected in HPL
| Is This Answer Correct ? | 1 Yes | 2 No |
Tell me something about Vitamin A test method by HPLC
why chiral compounds will rotate the plane polarized light in SoR.
What is difference between chemical Assay and Hplc assay?
difference between quality and quantity
what are the normalisation, internal standard and external standerd methods in HPLC.
if you have given a blank water how you analyse by general analytical techniques?
Which type of material analysis in GC using Theramal conditivity detector ?
difference between IR& FTIR
Why pH lies between 0 to 14 only
4 Answers PI Industries, Unimark Remedies,
on the basis of bcs class how to proceed the solubility?
What should be the level/ Percentage of Arsenic in Arsenic free zinc.
0 Answers FDC, Laurus, Pharmaceutical,
what is mean by 40 in the dissolution basket mesh size?
Organic Chemistry 
