What is use of LOD and LOQ in validtion and give range and in calculation why we use 3.3* LOD here why we use 3.3
Answers were Sorted based on User's Feedback
LOD is Limit of detection. We can dedect the peak but cant quantify
LOQ Limit od quaantification. We quantify also this is known as reporting thereshold.
During the RS analysis Below LOQ level we cant included in the total impurities calculation.
LOD and LOQ Determination three methods
1.S/N Ratio method
3 or 2 ratio for LOD
NLT 10 ratio for LOQ
2. Slope Method
Plot the linearity for active and impurities through serial dilution at lower level
Formula for LOD
Formula for LOQ
3. Visualization method
It will be using for only identification and TLC Method
|Is This Answer Correct ?||0 Yes||0 No|
Answer / kovida
LOD and LOQ are the smallest amount of the particular compound that can by detected and quantiified by using the developed HPLC method.
The signal to noise ratio is that minimum amount which when injected in HPLC it gives minimum detectable peak area.
The value of amount at this point is multiplied by 3 to get LOD
and by 10 to get LOQ value. In short LOD is the thrice value of minimum value and LOQ is ten times of minimum value that can be injected in HPL
|Is This Answer Correct ?||1 Yes||2 No|
How to prepare 10% HCl from 36.5% HCl solution.
What is the difference between Normal phase method & Reverse method in HPLC ? Define application of both method.
In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.
why kf factor coming 0.5
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?
What is the formula to determine the concentration of M of a solution given the % transmittance? Use %T = 43.7 as an example.
what is the meaning of linearity,principle of co-relation coefficient?
how can we calculate void volume?give its importance in brief
What is linearity in HPLC and how will you test?
what is end capping in hplc coulmn
what is the difference between c-8 and c-18 column
What is void volume & dead volueme