What is use of LOD and LOQ in validtion and give range and in calculation why we use 3.3* LOD here why we use 3.3

What is use of LOD and LOQ in validtion and give range and in calculation why we use 3.3* LOD here w..

Answer / kovida

LOD and LOQ are the smallest amount of the particular compound that can by detected and quantiified by using the developed HPLC method.

The signal to noise ratio is that minimum amount which when injected in HPLC it gives minimum detectable peak area.

The value of amount at this point is multiplied by 3 to get LOD
and by 10 to get LOQ value. In short LOD is the thrice value of minimum value and LOQ is ten times of minimum value that can be injected in HPL

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