Explain Homology modelling?



Explain Homology modelling?..

Answer / anubrata das

if the crystal structure of any protein is unavailable,
then one can use the tools of homology modelling to
determine the structure. the logic is that a similar
structure arises becuse of a similar sequence of amino
acids. for homology modelling to be accurate an identity
match of 70% is desirable.basically, one does a FASTA
search of A.A sequences in the PDB database (A.A sequences
whose structures are known), does a CLUSTAL alignment to
check for conserved residues. then the structure of the
unknown A.A sequence is built up on the basis of the
structure of the best matches in FASTA and CLUSTAL by
programs like LLOOP and HHPRED. this structure can be
visualized in programs like DEEPVIEW,Protein Explorer
etc.the EXPASY proteomics server is a good site to begin
with - http://swissmodel.expasy.org/

Is This Answer Correct ?    8 Yes 0 No

Post New Answer

More Bio Informatics Interview Questions

what is the use of Morphometrics?

1 Answers  


If you had 1,500 base pair pieces of random DNA and you have to know how many of them had homology to known genes, what would you do to determine that?

1 Answers  


How to Count number of nucleotide substitutions ?

0 Answers  


what is the difference between present and oldest drug discovery methods?

1 Answers   Thomson Reuters,


Some forms are books. All books are made of paper (a) Some forms are made of paper (b) Some forms are not made of paper (c) No forms are made of paper (d) None of the above.

1 Answers   Wipro,






How to caculate coordinates of each atoms on 3 D HP model?

0 Answers  


What is an alignment?

2 Answers  


A certain radioactive element A, has a half life = t seconds. In (t/2) seconds the fraction of the initial quantity of the element so far decayed is nearly (a) 29% (b) 15% (c) 10% (d) 45%

1 Answers   Wipro,


can u give me an example of a project you were involved with that illustrates your interest and skills in bringing people together?

0 Answers   AgriGold,


Integrate 3x + 5 / (x3-x2-x+1)?

0 Answers   Wipro,


In a balanced chemical equation (a) Atoms are conserved (b) Molecules are conserved (c) Moles are conserved (d) Reactant and product molecules are preserved.

1 Answers   Wipro,


When a bicycle is in motion, the force of friction exerted by the ground on the two wheels is such that it acts a) In the backward direction on the front wheel and in the forward direction on the rear wheel. (b) In the forward direction on the front wheel and in the backward direction on the rear wheel. (c) In the backward direction on both the front and rear wheels. (d) In the backward direction on both the front and rear wheels.

8 Answers   College School Exams Tests, IIM, IIT, JGK, Wipro,


Categories