Answer / anubrata das

if the crystal structure of any protein is unavailable,
then one can use the tools of homology modelling to
determine the structure. the logic is that a similar
structure arises becuse of a similar sequence of amino
acids. for homology modelling to be accurate an identity
match of 70% is desirable.basically, one does a FASTA
search of A.A sequences in the PDB database (A.A sequences
whose structures are known), does a CLUSTAL alignment to
check for conserved residues. then the structure of the
unknown A.A sequence is built up on the basis of the
structure of the best matches in FASTA and CLUSTAL by
programs like LLOOP and HHPRED. this structure can be
visualized in programs like DEEPVIEW,Protein Explorer
etc.the EXPASY proteomics server is a good site to begin
with - http://swissmodel.expasy.org/

Suppose the Blast search returned 100 hits. Of these, 17 were false positives and we knew that there were 165 sequences in the database which should have returned a hit with our sequence. How many false negatives were there, and what is the sensitivity and selectivity of Blast in this instance?

0 Answers  

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If the sum of the roots of the equation ax2 + bx + c=0 is equal to the sum of the squares of their reciprocals then a/c, b/a, c/b are in (a) AP (b) GP (c) HP (d) None of these

1 Answers   Wipro,

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A man speaks the truth 3 out of 4 times. He throws a die and reports it to be a 6. What is the probability of it being a 6? (a) 3/8 (b) 5/8 (c) 3/4 (d) None of the above

11 Answers   HDFC, Microsoft, MNC, TCS, Wipro,

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If the present age of my father is 39 yrs and my present age is x yrs, what is x?

1 Answers   Accenture,

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Suppose you used the NCBI Blast server to look for a match to a fruit-fly gene. Suppose further that the best alignment contained this section of an alignment: Hit from database: XXXXXXXXXXXXXXNFSTSQ User sequence: SLEAEAAPASISPSNFSSSQ What do the Xs signify?

0 Answers  

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What is PatternHunter?

1 Answers  

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What is an alignment?

2 Answers  

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What is the main idea of maximum parsimony in phylogenetic tree construction? What are the drawbacks?

2 Answers  

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NLO : RPS : : ? : ZXA (a) VUW (b) VTR (c) VTW (d) TRP

1 Answers   Wipro,

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you had 1,500 base pair pieces of random DNA and you wanted to know how many of them had homology to known genes, what would you do to determine that?

2 Answers  

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How long will a train 100m long traveling at 72kmph take to overtake another train 200m long traveling at 54kmph?

0 Answers  

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If a match from a sequence database search is reported to have an E-value of 0.0, should it be considered highly insignificant or highly significant?

3 Answers  

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