why we use TLC?
Answers were Sorted based on User's Feedback
Answer / d. sri krishna
TLC is a chromatographic separation technique. It is used
to separate different chemical like impurities in drug
substances.
Is This Answer Correct ? | 29 Yes | 1 No |
Answer / sateesh babu
1.IDENTIFYING UNKNOWN COMPOUND PRESENT IN MIXTURE
2.MONITORING PROGRESS OF REACTION
3.FOR RELATED SUBSTANCES DETERMINATION OR IMPURITY PROFILE DETERMINATION
Is This Answer Correct ? | 13 Yes | 2 No |
TLC means Thin layer chromatography.
Chromatography is a seperation techinique.Chromatography
is used to seperate components present in a mixture in to
individual components.Seperation of components is very
importent in pharmaceutical industry to know the nature of
the sample.So,we use TLC.
Uses of TLC :
1.To know the no of components present in a mixture.
2.To choose approprate solvent for column chromatography.
3.To moniter c-c seperation.
4.For reaction monitoring.(during in process of batch)
Procedure&principle:
One or more components are spotted on a adsorbent coated
chromatographic plate and this sample spotted plate is
placed in a solvent ie mobile phase and this mobile phase
is adsorbed by the plate slowly from downwards to upwards
due to capillary action agnist gravitational force.The
components with lower affinity towards mobile phase travels
slowly,and the components with high affinity towards mobile
phase will move faster.Thus components are seperated on a
tlc plate.
TLC PLATE:
The plate is the stationary phase.solvent is mobile
phase.TLC plate is nothing but silica gel like kiesel gel
60F254,silica gel 60F254,cellulose powder is spread on an
alluminium foil,or glass or plastic plate.
We can say more about TLC but not possible to say hear.
Refer web pages,you will get more information
Is This Answer Correct ? | 9 Yes | 1 No |
Answer / sanju
TLC is a simple, quick, and inexpensive procedure that
gives the chemist a quick answer as to how many components
are in a mixture. TLC is also used to support the identity
of a compound in a mixture when the Rf of a compound is
compared with the Rf of a known compound (preferrably both
run on the same TLC plate).
Is This Answer Correct ? | 6 Yes | 0 No |
Answer / vishvesh
TLC is a chromatography technique used to seperate non-volatile mixtures. It is used to determine the no. of components in a mixtures , the identity of compound & purity of of compounds.
Is This Answer Correct ? | 2 Yes | 0 No |
Answer / sanjay kumar
TLC is a chromatographic seperation technique it is used
the seperation of different chemical, TLC technique is
depend upon the molecular weight, related to retention
factor
Is This Answer Correct ? | 3 Yes | 4 No |
Answer / charu mehta
it is used for the separation of complex mixtures of chemically similar substances.
Is This Answer Correct ? | 0 Yes | 1 No |
how require to fix the linearity and accuracy cincentration range?
what is polarity and how to calculate
what is the difference between the Purity and Potency of drug substance
1 Answers Divis Laboratories, Graviti Pharmaceuticals,
EXPLAIN THE CALCULATION PART OF AN KF TITRATOR CALIBRATION WITH SUITABLE EXAMPLE
What is the column performance and column efficiency?
what is the meaning of linearity,principle of co-relation coefficient?
why we use x terra columns at higher ph ?
Why we compare unequal concentrated solutions in heavy metals test. For example comparing the test solution with 1 ppm lead standard and telling it's less than 20ppm?
1 Answers Micro Labs, Mistair,
Dear sir, This is R.Kadambavadani working in a pharma industry. while doing RS analysis of ibuprofen API, unknown peak eluting some times in the 0.6 Min RT. Kindly send me what is the exact reason behind that. also am using ACN the mobile phase.
what is the importence and role of LCMS and LCMS/MS and their applications? which type of compounds are analysis by lcms?
we are performed SOR for a particular product the limit is -6 to -10.We face particular bathes it is not meeting the specification.we performed diffrent instrument diffrent analyst we are getting diffrent diffrent results.solvent is dimethyl sulfoxide what could be the reasion
why ph electrode is storing in KCL solution?Why the pH max is 14.0?