Answer / d. sri krishna

TLC is a chromatographic separation technique. It is used
to separate different chemical like impurities in drug
substances.

Answer / sateesh babu

1.IDENTIFYING UNKNOWN COMPOUND PRESENT IN MIXTURE
2.MONITORING PROGRESS OF REACTION
3.FOR RELATED SUBSTANCES DETERMINATION OR IMPURITY PROFILE DETERMINATION

Answer / jagadeeshchandra

TLC means Thin layer chromatography.

Chromatography is a seperation techinique.Chromatography
is used to seperate components present in a mixture in to
individual components.Seperation of components is very
importent in pharmaceutical industry to know the nature of
the sample.So,we use TLC.


Uses of TLC :

1.To know the no of components present in a mixture.
2.To choose approprate solvent for column chromatography.
3.To moniter c-c seperation.
4.For reaction monitoring.(during in process of batch)

Procedure&principle:

One or more components are spotted on a adsorbent coated
chromatographic plate and this sample spotted plate is
placed in a solvent ie mobile phase and this mobile phase
is adsorbed by the plate slowly from downwards to upwards
due to capillary action agnist gravitational force.The
components with lower affinity towards mobile phase travels
slowly,and the components with high affinity towards mobile
phase will move faster.Thus components are seperated on a
tlc plate.

TLC PLATE:

The plate is the stationary phase.solvent is mobile
phase.TLC plate is nothing but silica gel like kiesel gel
60F254,silica gel 60F254,cellulose powder is spread on an
alluminium foil,or glass or plastic plate.

We can say more about TLC but not possible to say hear.
Refer web pages,you will get more information

Answer / sanju

TLC is a simple, quick, and inexpensive procedure that
gives the chemist a quick answer as to how many components
are in a mixture. TLC is also used to support the identity
of a compound in a mixture when the Rf of a compound is
compared with the Rf of a known compound (preferrably both
run on the same TLC plate).

Answer / vishvesh

TLC is a chromatography technique used to seperate non-volatile mixtures. It is used to determine the no. of components in a mixtures , the identity of compound & purity of of compounds.

Answer / sanjay kumar

TLC is a chromatographic seperation technique it is used
the seperation of different chemical, TLC technique is
depend upon the molecular weight, related to retention
factor

Answer / charu mehta

it is used for the separation of complex mixtures of chemically similar substances.

Answer / nandan

to separate and detect amino acids.

what should be the precision during technology transfer?

3 Answers   Glenmark,

tell me about aliphatic acid estimation by hplc which columns can we use

1 Answers   Dr Reddys,

Sulphated Ash incresed it will effect on product yield?

3 Answers   Biocon, Lupin,

From where require to take the RLD sample?

0 Answers  

plz tall me that any pharmacopeia and guideline is par meted to use 1.68,4.01,7.00,9.00,12.45 buffer for ph meter calibration

2 Answers   Torrent Pharma,

What are the terms used during Melting Range Analysis ?

2 Answers  

what is the maximum flow rate in prep hplc

1 Answers   Meril Life Science,

Why we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?

3 Answers   GVK,

U have 4 strengths have different coating meterial which one u chose to do forced degradation stady in validation

1 Answers   Reddy Labs,

What is the difference between low pressure gredient and high pressure gredient in hplc instrument?

4 Answers  

What is differences between the Instrument and Equipment

2 Answers   Aurobindo,

How can we konw that the HPLC column is endcapped? Is it possible to identify visually without COA?

2 Answers   JBCPL,