why we use TLC?
- 8 Answers
- 20051 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / d. sri krishna
TLC is a chromatographic separation technique. It is used
to separate different chemical like impurities in drug
substances.
| Is This Answer Correct ? | 29 Yes | 1 No |
Answer / sateesh babu
1.IDENTIFYING UNKNOWN COMPOUND PRESENT IN MIXTURE
2.MONITORING PROGRESS OF REACTION
3.FOR RELATED SUBSTANCES DETERMINATION OR IMPURITY PROFILE DETERMINATION
| Is This Answer Correct ? | 13 Yes | 2 No |
TLC means Thin layer chromatography.
Chromatography is a seperation techinique.Chromatography
is used to seperate components present in a mixture in to
individual components.Seperation of components is very
importent in pharmaceutical industry to know the nature of
the sample.So,we use TLC.
Uses of TLC :
1.To know the no of components present in a mixture.
2.To choose approprate solvent for column chromatography.
3.To moniter c-c seperation.
4.For reaction monitoring.(during in process of batch)
Procedure&principle:
One or more components are spotted on a adsorbent coated
chromatographic plate and this sample spotted plate is
placed in a solvent ie mobile phase and this mobile phase
is adsorbed by the plate slowly from downwards to upwards
due to capillary action agnist gravitational force.The
components with lower affinity towards mobile phase travels
slowly,and the components with high affinity towards mobile
phase will move faster.Thus components are seperated on a
tlc plate.
TLC PLATE:
The plate is the stationary phase.solvent is mobile
phase.TLC plate is nothing but silica gel like kiesel gel
60F254,silica gel 60F254,cellulose powder is spread on an
alluminium foil,or glass or plastic plate.
We can say more about TLC but not possible to say hear.
Refer web pages,you will get more information
| Is This Answer Correct ? | 9 Yes | 1 No |
Answer / sanju
TLC is a simple, quick, and inexpensive procedure that
gives the chemist a quick answer as to how many components
are in a mixture. TLC is also used to support the identity
of a compound in a mixture when the Rf of a compound is
compared with the Rf of a known compound (preferrably both
run on the same TLC plate).
| Is This Answer Correct ? | 6 Yes | 0 No |
Answer / vishvesh
TLC is a chromatography technique used to seperate non-volatile mixtures. It is used to determine the no. of components in a mixtures , the identity of compound & purity of of compounds.
| Is This Answer Correct ? | 2 Yes | 0 No |
Answer / sanjay kumar
TLC is a chromatographic seperation technique it is used
the seperation of different chemical, TLC technique is
depend upon the molecular weight, related to retention
factor
| Is This Answer Correct ? | 3 Yes | 4 No |
Answer / charu mehta
it is used for the separation of complex mixtures of chemically similar substances.
| Is This Answer Correct ? | 0 Yes | 1 No |
Identify problem faced when mass spectroscopy is used with HPLC system ?
0 Answers College School Exams Tests,
water is more polar than acetonitrile.but in reverse phase chromatography peaks elute earlier when we increse Acetonitrile.what is the reason.
What is the calibration of uv process and preparation
what is the use of tap density apparatus? why we calculate it? what is the difference between the bulk density and tap density?
1 Answers Alchemist, Alembic, Apex,
why we use a particular hplc column for a particular compound give reasons?please
0 Answers Dr Reddys, Indoco Remedies, Natco,
What is the name of the detector used in TOC?
what is the principal of malvern particle sizer & its used in pharma industry
why chiral separation?
Explain the relations between number of carbon atoms in alkanes and retention time ?
0 Answers College School Exams Tests,
what is the cleaning validation?
IN DISSOLUTION WE ARE USING A LETTER 'Q' WHAT IS MENT BY Q
5 Answers Reddy Labs, Torrent Pharma,
In Agilent HPLC calibration using Empower 3 Software, What is the criteria for GPV Baseline starting point? i.e., Either it can start from -0.00/+0.00 or any range is there like -0.05 to +0.05 or ......etc?
Organic Chemistry 
