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Answer Posted / jagadeeshchandra
TLC means Thin layer chromatography.
Chromatography is a seperation techinique.Chromatography
is used to seperate components present in a mixture in to
individual components.Seperation of components is very
importent in pharmaceutical industry to know the nature of
the sample.So,we use TLC.
Uses of TLC :
1.To know the no of components present in a mixture.
2.To choose approprate solvent for column chromatography.
3.To moniter c-c seperation.
4.For reaction monitoring.(during in process of batch)
Procedure&principle:
One or more components are spotted on a adsorbent coated
chromatographic plate and this sample spotted plate is
placed in a solvent ie mobile phase and this mobile phase
is adsorbed by the plate slowly from downwards to upwards
due to capillary action agnist gravitational force.The
components with lower affinity towards mobile phase travels
slowly,and the components with high affinity towards mobile
phase will move faster.Thus components are seperated on a
tlc plate.
TLC PLATE:
The plate is the stationary phase.solvent is mobile
phase.TLC plate is nothing but silica gel like kiesel gel
60F254,silica gel 60F254,cellulose powder is spread on an
alluminium foil,or glass or plastic plate.
We can say more about TLC but not possible to say hear.
Refer web pages,you will get more information
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