In GC, What does 'Column condensation test' mean by?
- 2 Answers
- 10184 Views
- Reddy Labs, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / sri krishna
Condensation Test
Use this test whenever injector or carrier gas contamination
problems are suspected (e.g., ghost peaks or erratic baselines).
* Leave the GC at 40-50�C for 8 or more hours.
* Run a blank analysis (i.e., start the GC, but with no
injection) using the normal temperature conditions and
instrument settings.
* Collect the chromatogram for this blank run.
* Immediately repeat the blank run as soon as the first
one is completed. Do not allow more than 5 minutes to elapse
before starting the second blank run.
* Collect the chromatogram for the second blank run and
compare it to the first chromatogram.
* If the FIRST chromatogram contains a substantially
larger amount of peaks and baseline instability, then that
is an indication that there is contamination upstream of the
capillary column (ie. contaminated inlet, dirty carrier gas,
etc.).
* If BOTH chromatograms contain few peaks or very little
baseline drift, it can be assumed that the carrier gas
and/or inlet are relatively clean.
* If BOTH chromatograms contain a significant amount of
noise and/or baseline drift, then that usually is an
indication that the detector or detector gases are contaminated.
| Is This Answer Correct ? | 8 Yes | 1 No |
Answer / guest
retention time per puss we r using mobile phase ph
| Is This Answer Correct ? | 0 Yes | 0 No |
wt is difference between assay and purity in hplc?
why we use glass fiber filters use in some situation?
What is difference between Potency & purity
in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?
In Stability testing if significant change occurs then what will be the action plan?
what is least count of a balance? How we find out this? Any formula for least count of Analytical balance?
13 Answers Acme Formulation, Analytical Solutions, Dr Reddys, Reddy Labs,
In Analytical method development related substance method when did follow w/w method when did follow area normalisation method
What is the difference between STANDARDIZATION and CALIBRATION of instrument like KF? why do we standardization of KF everyday? Why the KF factor being only 5.02 etc?
5 Answers AlpaMed, Vergo pharma research,
what is SOP?How can prepare?
In lcms analysis some times we get sodium and potassium adducts. if the compound is havine sodium ions how can we confirm wether sodium is from sample or it is a adduct?
2 Answers Teva Pharmaceuticals,
IS THERE ANY EQUIPMENT TO CHECK AND CALCULATE THE POLARITY OF A LIQUID?
why IR graph or spectra comes down ? Any reason
Organic Chemistry 
