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What is the procedure for Milk ring test?
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Answer / d.umamaheswari
A sample of whole milk is mixed well with a drop of the stained brucella antigen (a concentrated suspension of killed Br.abortus stained with hematoxyilin) and incubated in a water bath at 70c for 40-50 min. If anthibodies are present in the milk the bacilli are agglunited to form a blue ring at the top. If antibodies are absent no colored ring is formed, and the milk remains uniformly blue.
| Is This Answer Correct ? | 25 Yes | 3 No |
Answer / samantha bodnar
A milk test called Brucellosis Ring Test (BRT) is conducted
in dairies two to four times a year by testing a small
sample obtained from the creamery or farm milk tank for
evidence of brucellosis. Milk from each cow in the herd is
pooled and a sample is taken for testing. A suspension of
killed Brucella organisms is added to a small amount of
milk. If the test is positive, a bluish ring forms at the
cream line as the cream rises. All positive herds are
investigated. (from
http://www.cdfa.ca.gov/AHFSS/Animal_Health/Brucellosis_Info.html)
| Is This Answer Correct ? | 13 Yes | 0 No |
Answer / bharath
A special form of agglutination test done on the pooled milk
of many cows, usually entire herds, for the detection of
herds containing individuals infected with bovine brucellosis.
| Is This Answer Correct ? | 9 Yes | 4 No |
Answer / guest
An indirect enzyme-linked immunosorbent assay (ELISA) for
the detection of Brucella abortus antibodies in bovine bulk
milk samples was evaluated. About 31 individual milk
samples from B. abortus infected cows were diluted into
bulk milk from a brucellosis free herd. Individual milk
samples obtained from 96 negative or positive herds to
ELISA or Brucella ring test (BRT), were tested by ELISA.
All positive cows were bled and serum samples were tested
by the complement-fixation test (CFT) which was considered
the definitive test. A herd was considered infected if at
least, one cow was positive in the CFT. Four samples were
negative in the BRT at the dilution 1:10 but positive in
the ELISA. For samples positive in both tests, BRT titers
ranged from 1:10 to 1:480 while ELISA titers ranged from
1:10 to 1:3200.Using bulk milk samples, the sensitivity of
the ELISA (98.1%) was higher than the BRT (72.2%) but the
specificity of BRT (90.5%) was not statistically different
(P=1.0) from the ELISA (88.1%). The implications of the
results for brucellosis control are discussed.
| Is This Answer Correct ? | 9 Yes | 9 No |
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