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Answer Posted / d.umamaheswari
A sample of whole milk is mixed well with a drop of the stained brucella antigen (a concentrated suspension of killed Br.abortus stained with hematoxyilin) and incubated in a water bath at 70c for 40-50 min. If anthibodies are present in the milk the bacilli are agglunited to form a blue ring at the top. If antibodies are absent no colored ring is formed, and the milk remains uniformly blue.
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