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An indirect enzyme-linked immunosorbent assay (ELISA) for
the detection of Brucella abortus antibodies in bovine bulk
milk samples was evaluated. About 31 individual milk
samples from B. abortus infected cows were diluted into
bulk milk from a brucellosis free herd. Individual milk
samples obtained from 96 negative or positive herds to
ELISA or Brucella ring test (BRT), were tested by ELISA.
All positive cows were bled and serum samples were tested
by the complement-fixation test (CFT) which was considered
the definitive test. A herd was considered infected if at
least, one cow was positive in the CFT. Four samples were
negative in the BRT at the dilution 1:10 but positive in
the ELISA. For samples positive in both tests, BRT titers
ranged from 1:10 to 1:480 while ELISA titers ranged from
1:10 to 1:3200.Using bulk milk samples, the sensitivity of
the ELISA (98.1%) was higher than the BRT (72.2%) but the
specificity of BRT (90.5%) was not statistically different
(P=1.0) from the ELISA (88.1%). The implications of the
results for brucellosis control are discussed.
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