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How you will select the runtime in HPLC for a drug in
method development.
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Answer Posted / rahul
There are two three concerns to keep in mind when setting a
run time during method development.
1)Refer the degradation study data of the product.due to
this you come to know about the product beheviour with
method during stress conditions.
2)Confirm the impurity and main compund mass balance test
because of this you will assure about no further elution.
3)Run the HPLC for 5X to the column volume due to this you
can assure about and late elutions.
If with these experiments there is no elution after certain
time you can set the run time after 5 to 10mins after last
peak elution.
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