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what are the principles involved in thin layer
chromatography?

Answers were Sorted based on User's Feedback



what are the principles involved in thin layer chromatography?..

Answer / pooja

thin layer chromatography is based upon the principles of
column and partition chromatography.

seperation of a mixture in this case is acheived over a
thin layer of aluminium oxide or silica gel to which they
are adsorbed by different physical forces.

the resolved mixture may be extracted with suitable
solvents,later.

Is This Answer Correct ?    94 Yes 25 No

what are the principles involved in thin layer chromatography?..

Answer / prasanna

TLC is based on the principle of adsorption.the stationary
phase used in TLC is adsorbent such as silica gel coated
onto a inert solid support such as aglass plate.mobile phase
used is solvents of different polarity.when mixture of
components or sample is spotted on the prepared tlc plate
and placed in mobile phase they get seperated into
individiual components.silica coontains some free Si-OH
groups.these groups form h-bonds or other vanderwaal
interactions with the analyte components.thus adsorption
takes place.

Is This Answer Correct ?    24 Yes 4 No

what are the principles involved in thin layer chromatography?..

Answer / shivendra pratap singh

Thin layer cromatography is the seperation technique which
is based on the theory of capilary action.

Is This Answer Correct ?    41 Yes 27 No

what are the principles involved in thin layer chromatography?..

Answer / prashant

it is a physical method of seperation in whichthe
components to be seperated are distributed between 2 phases.
i.e.,1.stationary phase 2.mobile phase.

In tlc, if silica is used as stationary phase then it
principle is partition co-efficient

And if alumina is used then principle is adsorption.

The seperation in TLC involves distributing a mixture of 2
or more substances between a stationary and mobile phase.
seperation depends upon how much they adsorb on the
stationary phase versus how mwch they dissolve in the
mobile phase.

Is This Answer Correct ?    13 Yes 3 No

what are the principles involved in thin layer chromatography?..

Answer / radhika rao

Chromatography works on the principle that different
compounds will have different solubilities and adsorption
to the two phases between which they are to be partitioned.

Is This Answer Correct ?    12 Yes 4 No

what are the principles involved in thin layer chromatography?..

Answer / kailash a

This is sepration technique which are seprate out mixture
by capilary action on the silica gel coated plate.

Is This Answer Correct ?    15 Yes 8 No

what are the principles involved in thin layer chromatography?..

Answer / afaf

is a separation technique used to separate drug or mixture
of drugs by capillary action

Is This Answer Correct ?    17 Yes 11 No

what are the principles involved in thin layer chromatography?..

Answer / vishakha

thin layer chromatography is based on d principle of partition and coloum chromatography.in this techq. we generally use silica gel oo aluminium oxide as stationary phase over which solvent move from one pt. to another through capillary action and also due to the partition coeff. between stationary nd mobile phase.

Is This Answer Correct ?    9 Yes 3 No

what are the principles involved in thin layer chromatography?..

Answer / kenne hippolyte

Similar to other chromatographic methods TLC is also based
on the principle of separation. The separation depends on
the relative affinity of compounds towards stationary and
mobile phase. The compounds under the influence of mobile
phase (driven by capillary action) travel over the surface
of stationary phase. During this movement the compounds with
higher affinity to stationary phase travel slowly while the
others travel faster. Thus separation of components in the
mixture is achieved.

Once separation occurs individual components are visualized
as spots at respective level of travel on the plate. Their
nature or character are identified by means of suitable
detection techniques.

Is This Answer Correct ?    3 Yes 0 No

what are the principles involved in thin layer chromatography?..

Answer / nairuti patel

TLC Principle:Seperation of compounds from compound mixture based on their adsorption and desorption afiinites for stationary phase and mobile phase.

Is This Answer Correct ?    1 Yes 0 No

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