what are the principles involved in thin layer
chromatography?
- 12 Answers
- 38398 Views
- Aurobindo, Reddy Labs, I also Faced
- E-Mail Answers
Answer Posted / prasanna
TLC is based on the principle of adsorption.the stationary
phase used in TLC is adsorbent such as silica gel coated
onto a inert solid support such as aglass plate.mobile phase
used is solvents of different polarity.when mixture of
components or sample is spotted on the prepared tlc plate
and placed in mobile phase they get seperated into
individiual components.silica coontains some free Si-OH
groups.these groups form h-bonds or other vanderwaal
interactions with the analyte components.thus adsorption
takes place.
| Is This Answer Correct ? | 24 Yes | 4 No |
Post New Answer View All Answers
When it functions as a "second messenger", cAMP a) acts outside the cell to influence cellular processes. b) acts "second in importance" to AMP. c) activates all cytosolic protein kinases. d) activates the cAMP-dependent protein kinase.
Which equation gives the relation between specific rate (k) and temperature?
How can you determine the reaction, taking place at constant pressure delta (h)?
What is the structure of cscl and give the co-ordination number of Cscl?
Who were james watson, francis crik and maurice wilkins?
Which compound is involved in reducing levels of homocysteine in the blood?
How does the formation of the enzyme-substrate complex? Explain the reduction of the activation energy of chemical reactions?
What is addition reaction?
Name the first synthetic organic compound. Who proposed it and from which compound?
Why fe3+ ion is more stable than fe2+ ion is?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
What are respectively some remarkable functions of myosin, cd4, albumin, keratin, immunoglobulin, reverse transcriptase, hemoglobin, and insulin?
Glycolysis is A) C6H12O6 --> 2C2H5OH + 2CO2 B) C6H12O6 --> 2CH3H4O3 + 4H c) C3H4O3 + NAD --> C2H5OH + CO2 NAD D) C6H12O6 + 6H20 --> 6CO2 + 6H2O
What are enzyme cofactors?
Explain threshold energy?
