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what are the principles involved in thin layer
chromatography?
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Answer Posted / prasanna
TLC is based on the principle of adsorption.the stationary
phase used in TLC is adsorbent such as silica gel coated
onto a inert solid support such as aglass plate.mobile phase
used is solvents of different polarity.when mixture of
components or sample is spotted on the prepared tlc plate
and placed in mobile phase they get seperated into
individiual components.silica coontains some free Si-OH
groups.these groups form h-bonds or other vanderwaal
interactions with the analyte components.thus adsorption
takes place.
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