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what are the principles involved in thin layer
chromatography?
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Answer Posted / kenne hippolyte
Similar to other chromatographic methods TLC is also based
on the principle of separation. The separation depends on
the relative affinity of compounds towards stationary and
mobile phase. The compounds under the influence of mobile
phase (driven by capillary action) travel over the surface
of stationary phase. During this movement the compounds with
higher affinity to stationary phase travel slowly while the
others travel faster. Thus separation of components in the
mixture is achieved.
Once separation occurs individual components are visualized
as spots at respective level of travel on the plate. Their
nature or character are identified by means of suitable
detection techniques.
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