Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
- 60 Answers
- 187698 Views
- Arvind, Aurobindo, Cipla, Reddy Labs, Shasun Chemicals, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / pranav desai
1#It is primary reference stanard.
2#It is suggested in US,IcH Pharmacopiea.
3#It is 2 Mixima and 1 Minima
(205nm,273nm)(245nm)
4#it is eslyli soluble in solvent.
5# it is less carry over.
| Is This Answer Correct ? | 1 Yes | 2 No |
Answer / ravi kiran
it will detect at dual wave length and easily available in
market not degrade
| Is This Answer Correct ? | 0 Yes | 1 No |
Answer / satishchandra m.patel
2 maximas
and 1 minima
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
also suggested in pharmacopiea
caffeine easily available in marketIt is easily soluble in
water
| Is This Answer Correct ? | 1 Yes | 3 No |
Answer / k.g.gupta dr.reddys
Most of the Pharmaceutical products are analysing in the
range of 200-300nm. Caffiene is having 2maxima and 1
minima.It is very stable molecule.
| Is This Answer Correct ? | 2 Yes | 4 No |
Answer / b.naga vara lakshmi
1.IT SHOW MAXIMUM WAVELENGTH, AND ALSO PRIMARI REFERENCE
STANDARD,
2.IT PHYSICALLY STABLE IN NATURE AND USE LONG TIME,
3.IT IS FREELY SOLUBLE IN ANY SOLVENT.
| Is This Answer Correct ? | 0 Yes | 2 No |
Answer / suresh nv
easily avalable inmarket give good resolution
| Is This Answer Correct ? | 3 Yes | 7 No |
Answer / shivakumar
Caffeine is used for HPLC calibration in detector wavelength
accuracy test just because.
-it is completely soluble in water.
-it have a range of absorption wavelengths.
max at 205nm minima at 245nm and a maxima at 273nm.
- more over it is very stable for long time even in
solution(water).
| Is This Answer Correct ? | 3 Yes | 8 No |
Answer / vikram kodre
along with above answers very important think is caffeine
extracted from nature, so it get in highly pure form.
| Is This Answer Correct ? | 15 Yes | 25 No |
Answer / ajay
caffiene in needle shape easily dissolve in methanol &
methanol is very chip
| Is This Answer Correct ? | 11 Yes | 44 No |
Answer / vaibhav jain
Its somethng chromphore group which is caffeine having i m
nt surely tell bt whnvr i m sure i wl post answer
| Is This Answer Correct ? | 28 Yes | 104 No |
Why forced degradation studies are performed in hplc method validation?
why kf factor coming 0.5
give tha clarity about peak purity concept
3 Answers Aurobindo, Reliance Life Sciences,
IN pH CALIBRATION,CALIBRATION IS DONE FOR pH METER OR ELECTRODE?HOW WILL YOU JUDGE THAT THE METER TO BE CALIBRATED WITH 4,7,9.2 BUFFERS?EXPLAIN TEMPERATURE EFFECTS ON pH.
What is Threshold in hplc?
how you start the solubility study?
How we can determine OOT limit in Stability
why we are analyzing purity on HPLC why not in gc
3 Answers Aurobindo, Reddy Labs,
on what basis SOP's can be changed? IF mobile phase ratio is 70:30 to how much % can we change the ratio in HPLC process
What is linearity in hplc and how to calibration
In Force degradation Study Compound is not degrade in any condition , also in higher stress what can i do ?
from where i get mortar pastle for glass bottle crush? it's required for testing of it?
Organic Chemistry 
