Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test

Answer Posted / vikram kodre

along with above answers very important think is caffeine
extracted from nature, so it get in highly pure form.

Is This Answer Correct ?    15 Yes 25 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

Principle of single pan analytical balance

4614


[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?

324


in stress study if your api not soluble in hcl naoh h2o2 then what require to do?

350


Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...

1237


As per ICH related substances stability trend limit from initial to shelf life

2692






How we choose the mobile phase for method development.?

672


why xterra column require to use at higher ph?

449


What is the difference between Discriminating media and DPDM(Dissolution Profile with Different Media)

6957


How to establish relative response factor for hplc. Why it is required?

720


What is the formula for relative diffrence for standard solution in solution stability in validation?

321


In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?

1471


why multimedia dissolution require to do?

324


how pda detector works over uv?

302


why require the ph, buffer during hplc mobile phase?

419


A ha tc is actual defination of retention time?

1077