Interested to Buy Any Domain ? << Click Here >> for more details...
BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.4 : The resolution of separation, Rs for chromatography is given by the formula Rs = (difference in retention time) / (average width at the base). In a chromatogram, 3 peaks a, b and c are found. Average widths W at the bases of the solutes are : Wa = 20 s, Wb = 40 s, Wc = 30 s. Resolutions of separation, Rs for solutes b and c in comparison to a are 2 and 4 respectively. The differences in retention times T for b and c in comparison to a are (Tb - Ta) and (Tc - Ta), Ta = Tc - Tb : (a) Form 2 equations involving Rs as a function of Wa, Wb, Wc, Ta, Tb and Tc. (b) Find the values of Ta, Tb and Tc.
- 1 Answers
- 2290 Views
- I also Faced
- E-Mail Answers
BIOCHEMICAL ENGINEERING INSTRUMENTATION - ANSWER 29.4 : Reference formula Rs = (difference in retention time) / (average width at the base) is used. (a) First equation : Rs = (Tb - Ta) / [ (Wa + Wb) / 2 ] = 2 (Tb - Ta) / (Wa + Wb). Second equation : Rs = (Tc - Ta) / [ (Wa + Wc) / 2 ] = 2 (Tc - Ta) / (Wa + Wc). (b) Substitute Ta = Tc - Tb, Wa = 20 s, Wb = 40 s and Wc = 30 s into first equation and second equation. First equation : Rs = 2 = 2 [ Tb - (Tc - Tb) ] / (20 + 40) = (2 Tb - Tc) / 30, 2 Tb - Tc = 60. Second equation : Rs = 4 = 2 [ Tc - (Tc - Tb) ] / (20 + 30) = Tb / 25, Tb = 100 s. Substitute Tb = 100 s into first equation gives 2 Tb - Tc = 2 x 100 - Tc = 200 - Tc = 60, then Tc = 200 - 60 = 140 s. Then Ta = Tc - Tb = 140 - 100 = 40 s. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
| Is This Answer Correct ? | 0 Yes | 0 No |
Explain the steps taken to operate a tank-blanketing valve?
did sulphur been a byproduct after the combustion of LPG and Natural Gas
Why does catalytic converter used in an automobile?
ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.22 : An engineering company intends to produce a small piece of biochemical instrument for sales. Let A = overall fixed cost of production, B = variable cost of production per unit, C = selling price per unit, D = quantity of unit produced. Breakeven Analysis is used where revenue = cost. (a) Explain the role of Breakeven Analysis by using A, B, C and D. (b) Find the value of Contribution Margin in term of A, B, C and / or D. (c) At the breakeven point where revenue = cost, derive an equation of D as a function of A, B and C.
Mention some of the specialized crushing methods?
why back washing is performed in DM water plant?
What are the greenhouse gases in earth's atmosphere?
Explain the advantages of using a ball mill over other conventional methods of crushing?
what is difference between precipitation and crystalisation
what is meaning of pid how it is useing controlers
testing formula for oxygen by ammonium chloride,ammonium hydroxide and copper
How can wet carbon dioxide be responsible for a corrosion problem in iron-containing metals?
-
Civil Engineering (5086) - Mechanical Engineering (4456)
- Electrical Engineering (16639)
- Electronics Communications (3918)
- Chemical Engineering (1095)
- Aeronautical Engineering (239)
- Bio Engineering (96)
- Metallurgy (361)
- Industrial Engineering (259)
- Instrumentation (3014)
- Automobile Engineering (332)
- Mechatronics Engineering (97)
- Marine Engineering (124)
- Power Plant Engineering (172)
- Textile Engineering (575)
- Production Engineering (25)
- Satellite Systems Engineering (106)
- Engineering AllOther (1379)
