A blastomere was removed from an 8-cell human embryo, and
its beta-globin gene was amplified by PCR. The beta-globin
DNA was treated with an enzyme that cuts on either side of
and within the normal allele (producing a small fragment),
but not within the sickle cell allele (producing a large
fragment). Electrophoresis of the treated DNA from the
blastomere demonstrated the presence of both a large and
small fragment derived from the beta-globin genes. How
should you interpret this test?
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Cystic fibrosis (CF) occurs with a frequency of about 1/2500 Caucasian newborns and is inherited as an autosomal recessive. A woman had an older sister die from complications of this disease. CF is not present among relatives of her husband. Both the woman and her husband have normal phenotypes. What is the chance this couple will have a CF child
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Are there additional resources / individuals that you can recommend for my paper?
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