what is the difference between MUFA and PUFA?

5 Answers   Matrix,

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What role do disulfide bonds play in protein folding, and in protein stability?

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How does temperature affect the action of enzymes upon their substrates?

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How does hplc differ from normal column chromatography, and what are its advantages?

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What is the difference between Gram positive & Gram negative bacteria based on its cell contents?

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which solvent mixture is normally used in paper chromatography?

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On what structural level of the enzyme (primary, secondary, tertiary, or quaternary) does the enzyme-substrate interaction depend?

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One characteristic of the dna molecule is its replication capability. What are the consequences of failures during dna replication?

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What is the anhydride of n2o5?

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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

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how bpg acts as a haemoglobin regulator?

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how do u differentiate pseudoglobulins and euglobulins on the basis of solubility?

1 Answers  

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