Interested to Buy Any Domain ? << Click Here >> for more details...
How do you find the D and F value for Biological indicator,
in the autoclave?
- 1 Answers
- 10196 Views
- Cipla, I also Faced
- E-Mail Answers
Answer / mohit bibra
THE AUTOCLAVE USED FOR THE STERLISATION METHODS IS SET AT
TEMPERATURE OF 121.1◦C.
1)TO FIND THE F VALUE(WHICH REPRESENTS A MEASURE OF THE
CAPACITY OF A HEAT PROCESS TO REDUCE THE NUMBER OF SPORES
OR VEGETATIVE CELLS OF A GIVEN ORGANISM PER CONTAINER)OF AN
BIOLOGICAL INDICATOR THE NUMBER OF THE CELLS IN THE INITIAL
POPULATION BEFORE KEEPING IN THE AUTOCLAVE AND THE NUMBER
OF THE CELLS IN THE FINAL POPULATION AFTER TAKING OUT OF
THE AUTOCLAVE ARE COUNTED USING A HEMATOCYTOMETER AND THE F
VALUE IS CALCULATED BY THE FORMULA
F0 = Dr (log a − log b),
2)TO CALCLATE THE D VALUE(WHICH REPRESENTS THE TIME
REQUIRED TO KILL THE 90% OF THE POPULATION)THE ININTIAL
NUMBER OF THE CELLS AND THE FINAL NUMBER OF THE CELLS ARE
COUNTED AS STATED ABOVE AND THEN A SEMI-LOGARITHMIC GRAPH
BETWEEN THE NUMBER OF THE CELLS(ON THE Y-AXIS LOGARTHMIC
SCCALE)AND TIME(ALONG THE X-AXIS ON NORMAL SCALE)IS
PLOTED.THE POINT AT WHICH THE 90% OF THE POPULATION DIES
NOTE THAT TEMPERATURE FRROM THE GRAPH WHICH REPRESENS THE D-
VALUE.
| Is This Answer Correct ? | 18 Yes | 3 No |
What is the difference between transformation and transduction?
How are heat labile fluids such as serum and antibiotic solutions sterilized?
How to prepare and store agar?
Explain the Quantitative PCR for Intestinal Samples?
How to sterlize yeast on mold plates?
what is Selective Media of desoxycholate (DOC) agar ?
Describe the Immunofluorescent staining of Actinomycetes?
can you send me the previous years drug inspector exam model papers of andhrapradesh
Difference between respiration and digestion
Why we are maintaining the autoclave temperature at 121degree centigrade and pressure at 15lbs? Why we are not maintaining the temperature and pressure above or below 121degree centigrade and 15lbs? higher temperature also kills microbes. then why we are not maintaining it above 121degree? what is the reason?
Name the chemical compound which is a surface disinfectant acceptable for use on treatment areas after therapy on a suspected hepatitis B carrier?
Compare the two Viruses Baculovirus vs. Flavivirus ?
Micro Biology 
