Explain the INS1 cell culture and transfection?
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Answer / Sandeep Singh
INS-1 cells are a rat insulinoma cell line, used in research for studying pancreatic beta cells and diabetes. To culture INS-1 cells, they are typically maintained in RPMI-1640 medium supplemented with FBS, penicillin, streptomycin, and L-glutamine. For transfection, cells are usually transfected using methods such as electroporation or lipofection with plasmid DNA containing the gene of interest.
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