Why we use uracil and caffeine in hplc calibration

Answers were Sorted based on User's Feedback



Why we use uracil and caffeine in hplc calibration..

Answer / naveen

both are primary standards with high purity and have known wavelength maximum. So, can be used for calibration of HPLC.

Is This Answer Correct ?    21 Yes 5 No

Why we use uracil and caffeine in hplc calibration..

Answer / mahdi

because because sharp absorbance bands and good solution stability of caffeine, this substance used for calibration of UV detector.

Is This Answer Correct ?    11 Yes 2 No

Why we use uracil and caffeine in hplc calibration..

Answer / mahesh patil

Because caffeine gives two maxima and one minima and it is more stable compound.

Is This Answer Correct ?    0 Yes 0 No

Why we use uracil and caffeine in hplc calibration..

Answer / bharat amipara

Its a stable small difference in temperature and atmosphere variation

Is This Answer Correct ?    0 Yes 0 No

Why we use uracil and caffeine in hplc calibration..

Answer / sridurga

caffine and uracil will detect through out uv region 

Is This Answer Correct ?    2 Yes 3 No

Why we use uracil and caffeine in hplc calibration..

Answer / mahendra vaghela

Both compound are respond on multi wavelength.

Is This Answer Correct ?    0 Yes 1 No

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