Why we use uracil and caffeine in hplc calibration
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Answer / naveen
both are primary standards with high purity and have known wavelength maximum. So, can be used for calibration of HPLC.
| Is This Answer Correct ? | 22 Yes | 6 No |
Answer / mahdi
because because sharp absorbance bands and good solution stability of caffeine, this substance used for calibration of UV detector.
| Is This Answer Correct ? | 11 Yes | 2 No |
Answer / mahesh patil
Because caffeine gives two maxima and one minima and it is more stable compound.
| Is This Answer Correct ? | 2 Yes | 0 No |
Answer / bharat amipara
Its a stable small difference in temperature and atmosphere variation
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / sridurga
caffine and uracil will detect through out uv region
| Is This Answer Correct ? | 2 Yes | 3 No |
Answer / mahendra vaghela
Both compound are respond on multi wavelength.
| Is This Answer Correct ? | 0 Yes | 1 No |
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Organic Chemistry 
