Why we use uracil and caffeine in hplc calibration
Answers were Sorted based on User's Feedback
Answer / naveen
both are primary standards with high purity and have known wavelength maximum. So, can be used for calibration of HPLC.
| Is This Answer Correct ? | 22 Yes | 6 No |
Answer / mahdi
because because sharp absorbance bands and good solution stability of caffeine, this substance used for calibration of UV detector.
| Is This Answer Correct ? | 11 Yes | 2 No |
Answer / mahesh patil
Because caffeine gives two maxima and one minima and it is more stable compound.
| Is This Answer Correct ? | 2 Yes | 0 No |
Answer / bharat amipara
Its a stable small difference in temperature and atmosphere variation
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / sridurga
caffine and uracil will detect through out uv region
| Is This Answer Correct ? | 2 Yes | 3 No |
Answer / mahendra vaghela
Both compound are respond on multi wavelength.
| Is This Answer Correct ? | 0 Yes | 1 No |
In chromatograpbic purity 90% is there, in assay 70% is there. How will you prove remaining 30%.
WHAT IS THE USAGE OF REFRACTIVE INDEX IN PHARME FIELD
If suppose 10 methods of dissolution given in pharmacopoeial for single content product then which method out of 10? or all 10 require to follow?
Why Peak Purity is not determined in GC analysis?
What is polymorphism?which type of compounds exhibits polymorphism?
what is the difference between capillary and glass column in GC
why 0.005 M sulphuric acid is used to calbrate spectrophotometer and why caffiene is used for calibrating hplc
2 Answers GVK, Torrent Pharma,
What is the difference between void volume and delay volume in HPLC?
Please do not join if u got offer in oncotherapies ltd (STRIDESARCOLAB)especially Mr.Mahesh Bhatt team because job is not secure his team and doesn't have confidence on him becareful this offer?
Difference between related substances & impurities
give tha clarity about peak purity concept
3 Answers Aurobindo, Reliance Life Sciences,
in dissolution if one bowl got 70 percent 2nd bowl got 80 percent and 3rd bowl got 90 percent then how proceed?