How to Prepare cultures of known concentration ?



How to Prepare cultures of known concentration ?..

Answer / Shekhar Singh

Preparing cultures of known concentration involves diluting a microorganism in a sterile medium to achieve a specific number of cells per unit volume. This can be done using a process called serial dilution, where the initial culture is serially diluted and plated on an agar plate. By counting the colonies that grow after incubation, the concentration of the initial culture can be determined.

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