Answer Posted / biomaster
The ultracentrifuge method for determining the molecular
weights of proteins was developed by svedberg.
in this method protein molecules are subjected to
gravitational forces greater in magnitude than thermal
forces whereby causing them to diffuses.protein molecules
are larger in size called macromolecules.
under high centrifugal forces these molecules can be made
to sediment towards one end of a centrifuge tube.
The equation derived by svedberg for the molecular weight
of a protein is
M=RTS/D(1-Vp)
where R=gas constant
T=absolute temperature
s=sedimentation constant
D=diffusion constant
V=partial specific volume ofprotein
p=density of solvent
PRINCIPLE OF ULTRACENTRIFUGE IS "For most proteins however
the value of V is in the range of 0.70 to 0.75".
| Is This Answer Correct ? | 53 Yes | 16 No |
Post New Answer View All Answers
Differentiate between gram +ve and gram -ve bacteria.
To calculate the e.m.f of the cell when does the nelsons equation is use?
What is the importance of the primary structure of a protein?
What are hydrides of boron called?
Name the instrument used for sequencing proteins automatically?
Why does the pH of the blood decrease in a person who has digested trematol?
How does hplc differ from normal column chromatography, and what are its advantages?
Which enzymes in cows allow them to digest cellulose?
What is the structure of cscl and give the co-ordination number of Cscl?
can i used antimony potassium tartrate against potassium tartrate in Fehling B solution ?
Bleaching powder is obtained when cl2 is passes through which inorganic compound?
What is the shape of molecules SF6 and IF7?
What are some factors that can lead to protein denaturizing?
In water molecule the bond angle decreases from 109.28 to 104.5, why this does happens?
Which type of chemical bond maintains the pairing of each chain in the dna molecule?
