Interested to Buy Any Domain ? << Click Here >> for more details...
Why we are maintaining the autoclave temperature at
121degree centigrade and pressure at 15lbs? Why we are not
maintaining the temperature and pressure above or below
121degree centigrade and 15lbs? higher temperature also
kills microbes. then why we are not maintaining it above
121degree? what is the reason?
- 4 Answers
- 38986 Views
- Biocon, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / kk pandey
bcoz the approx 120 degree is the highest temp which the spore
of thermophilic bacteria (Bacillus sterothermophilus)can
withstand.
Hence for autoclaving temp is kept 121 so as to kill even the
spores is killed.
Now why the temp is not increased from 121 is bcoz at higher
temp the media, or other nutritious material which u r
autoclaving can get denatured.
| Is This Answer Correct ? | 110 Yes | 11 No |
Answer / kk pandey
bcoz the approx 120 degree is the highest temp which the spore
of thermophilic bacteria (Bacillus sterothermophilus)can
withstand.
Hence for autoclaving temp is kept 121 so as to kill even the
spores is killed.
Now why the temp is not increased from 121 is bcoz at higher
temp the media, or other nutritious material which u r
autoclaving can get denatured.
| Is This Answer Correct ? | 43 Yes | 10 No |
Answer / atul sharmz
S.thermophillus bateria is heat stable bacteria, this one
also killed at 121 degree c and 15 lb p by coagulate the
protein of bacteria. thats by we maintaine the temp 121 or
15lb pressrue. At higher temperature nutrient
denatured.
| Is This Answer Correct ? | 25 Yes | 8 No |
Answer / vamsi
we are maintaining auto clave temperature at 121degree centigrade at 15lbs pressure this is for complete destroy spores.If we keep above 121degree centigrade we have to decrease the time that is 125degree centigrade for 10minutes and 130degree centigrade for 3minutes. If we keep above 130degree centigrade there the nutrients will denatured.
| Is This Answer Correct ? | 23 Yes | 10 No |
what is lociculture
What is fecal coliform?
What is the temperature and time employed to sterilize the articles in hot air oven?
Describe the Reed and Muench Method ?
Differentiate between doubling time and generation time?
Describe about diatoms?
How hot and long is Pasteurization?
Who introduced acid fast staining?
What are the cardinal signs of inflammation ?
You suspect microscopic grains on a slide that you exposed to the outside air to be pollen. To test this, you place a drop of a sugar solution on the slide with the grains. If this is pollen, what will happen?
What are the Antibiotic working concentrations?
How are stains classified?
Micro Biology 
