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Why we are maintaining the autoclave temperature at
121degree centigrade and pressure at 15lbs? Why we are not
maintaining the temperature and pressure above or below
121degree centigrade and 15lbs? higher temperature also
kills microbes. then why we are not maintaining it above
121degree? what is the reason?
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Answer Posted / vamsi
we are maintaining auto clave temperature at 121degree centigrade at 15lbs pressure this is for complete destroy spores.If we keep above 121degree centigrade we have to decrease the time that is 125degree centigrade for 10minutes and 130degree centigrade for 3minutes. If we keep above 130degree centigrade there the nutrients will denatured.
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