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Why we are maintaining the autoclave temperature at
121degree centigrade and pressure at 15lbs? Why we are not
maintaining the temperature and pressure above or below
121degree centigrade and 15lbs? higher temperature also
kills microbes. then why we are not maintaining it above
121degree? what is the reason?
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Answer Posted / kk pandey
bcoz the approx 120 degree is the highest temp which the spore
of thermophilic bacteria (Bacillus sterothermophilus)can
withstand.
Hence for autoclaving temp is kept 121 so as to kill even the
spores is killed.
Now why the temp is not increased from 121 is bcoz at higher
temp the media, or other nutritious material which u r
autoclaving can get denatured.
| Is This Answer Correct ? | 110 Yes | 11 No |
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