What is 'polymerase chain reaction'?
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Answer / sunilreddy.vancha
A technique for amplifying DNA sequences in vitro by
separating the DNA into two strands and incubating it with
oligonucleotide primers and DNA polymerase.
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Answer / d.jeeva jothi
The Polymerase chain reaction (PCR) is an effective
technique that helps produce multiple copies of a target DNA
sequence.
It amplifies DNA that are either too small, or too degraded.
Thus it is possible to analyse DNA got in minute quantities
in places like a drop of blood at a crime scene or a cell
from an extinct dinosaur.
Thermal cycler is an instrument which is used to run the PCR
experiments. PCR was discovered by Kary Mullis in 1984.
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Answer / atul gaur
+with the help of different temperatures
1> denaturation 94 c
2> annealing 54 c
3> synthesis 74 c
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