Explain activation energy?
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What is xanthoproteic test?
What are the structural and stability consequences of electron delocalization in amides (peptide bonds)?
To calculate the e.m.f of the cell when does the nelsons equation is use?
How the NUCLEOSIDE ANALOGUES ARE USED AS DRUGS?
who discovered the aminoacid sequence of the insulin?
How is glycogen synthesized?
WHAT ARTE CHARGAFF'S EQUIVALENCE RULES?
Do the phosphate and the pentose groups give homogeneity or heterogeneity to the nucleic acid chains? Supported by that which of those groups is expected to participate in the highly diverse and heterogeneous genetic coding, i.e., Which of those groups is the basis of the information for protein production?
How do you measure the pollution in water
I understand that urine can act as a reagent to break disulfide bonds? I am particularly interested in bonds between cysteines. and or any other amino acids.
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what is meant by panhypopituitarism?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line