what is the significant of pH in HPlc?
- 5 Answers
- 13642 Views
- Orchid, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
selecting the pH of M.P. is important to get credible
retention time and is based on the pKa value, polar or
non-polar nature of the analyte.
| Is This Answer Correct ? | 33 Yes | 1 No |
Answer / roshan bhaskar
pH can change the hydrophobic character of the analyte. For this reason most methods use a buffering agent, such as sodium phosphate, to control the pH. Buffers serve multiple purposes: control of pH, neutralize the charge on the silica surface of the stationary phase and act as ion pairing agents to neutralize analyte charge.
| Is This Answer Correct ? | 2 Yes | 1 No |
Answer / pawan gupta
pH is the main role in M.P. pH depend retention time &peaks Sharp
| Is This Answer Correct ? | 0 Yes | 0 No |
What is the role of SO2,I2, Methanolic hydroxide and any base in Karlficher titration
2 Answers Medley Pharmaceuticals,
why chiral separation?
While performing TOC sst analysis Zero shift disabled & sample analysis zero shift enabled why?
Explain what is a base line?
What is the difference between chiral cel OD-H & OD-RH ?
What is the Deference between Bulk density & Tap density ?
2 Answers Hikma Pharmaceuticals, Neuland, Reddy Labs,
how can you determine a compound acidic or basic?
how to calibrate hplc & gc
what is biorelivent dissolution media?
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?
WHY pKa value is more important than pH value of the mobile phase in HPLC?
What is Specificity ?
Organic Chemistry 
