Interested to Buy Any Domain ? << Click Here >> for more details...
-
Biological Sciences (4520) - Physics (1496)
- Chemistry (2582)
- Health Pharma (592)
- Medicine (1862)
- Agriculture (179)
- Mathematics (232)
- Statistics (73)
- General Science (496)
- Physical Science (31)
- Astronomy (194)
- Political Science (71)
- Environmental Science (23)
- Sciences AllOther (161)
Why we are performing wavelength accuracy at lower and higher wavelengths in HPLC calibration
3 14936what is the acceptable limits for coliforms ,total bacterial Count ,yeast and mould in drinking water
9 24981Why is the RBC called as a cell even though it dont have all the characteristic features of a complete cell?
11 20943What will be in case stability sampleWhich have batch but distribute in three country.In tath case< we can put the sample for all country, whenevr all have same temp(Zone)
5 9734What is meant by Chiral compounds .Give exmaples of optically active compounds in which there are no chiral carbon atom.
1 8360
How stars produce energy?
What factors can change the pKa value of an amino acid residue, and in which direction?
Which element in 1A group does not form peroxides?
How to reduce cell clumping for immunocytostaining ?
Which is the autotrophic group responsible for the production of most part of the molecular oxygen of earth?
What modification is required for the atomic theory in order to explain the existence of isotopes, and modern view of atomic nuclei and electrons?
Explain in brief about the principles of quantal analysis in the peripheral and central nervous system.
Did india use the special safeguard provisions available in the agreement on agriculture?
What are hormones?
Give an account on Virus de-envelopment ?
What is type II hypersensitivity?
What are sv and sh waves?
What are plate hyperthermophiles ?
What is the basic philosophy of health in ayurveda?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
