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Question 88 - In the calculation of the growth of bacteria, colony forming unit (CFU) in serial dilution is used. In a laboratory, viable count assay is used to estimate CFU. Formula applied is CFU / mL = (number of colonies x dilution) / (amount plated, in unit mL). Acceptable plate count is either between 20 and 200 or between 30 and 300 according to 2 different references. A wastewater sample of 200 ml is added to and mixed with 1.8 L of sterile water. Another 200 ml of the mixture is added to and mixed with 1.8 L of sterile water. (a) Calculate the dilution of first mixture and the dilution of the second mixture. (b) 100 microlitres of wastewater samples from the first mixture and the second mixture are placed separately on 2 different alga plates. The first plate has 250 colonies and the second plate has 23 colonies. Calculate the average CFU / mL.

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Question 76 - Let C% be the fractional crystallinity, Rs = density of sample, Ra = density of amorphous form and Rc = density of crystalline form. In a polymer, these unknowns could be related by the equation C% = (Rc / Rs) (Rs - Ra) / (Rc - Ra). (a) Find the equation of Rc as a function of C%, Rs and Ra. (b) Two samples of a polymer, C and D exist. For sample C, C% = 0.513 when Rs = 2.215 unit. For sample D, C% = 0.742 when Rs = 2.144 unit. Both samples C and D have the same values of Ra and Rc. Find the values of Ra and Rc in 6 decimal places.

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A 1.5 weight % aqueous salt solution is concentrated to 4 weight % in a single-effect evaporator. The feed rate to the evaporator is F = 7500 kg / h and the feed is at 85 degree Celsius. The evaporator operates at 1 bar. By assuming that only pure solvent of water exists in the form of vapor from the feed, calculate the flow rate of such vapor V.

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QUANTUM COMPUTING - EXAMPLE 32.6 : (a) Let H | 0 > = 0.707 ( | 0 > + | 1 > ), H | 1 > = 0.707 ( | 0 > - | 1 > ). Find the values for H | 0 > + H | 1 > and H | 0 > - H | 1 >. (b) In quantum computing, a qubyte is a quantum byte, or 8 quantum bits, a sequence processed as a unit. A qubit is a quantum bit. According to Alexander Holevo in his theorem, n qubits cannot carry more than n classical bits of information. What is the maximum amount of classical bits of information that can be carried by 1 qubyte.

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Can anyone tell me how to calculate the quantity of a third component added in an azeotropic batch distillation of a known quantity of azeotropic mixture?

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Question 35 – A mixture consists of benzene (B), toluene (T) and xylene (X). At a temperature of 353 K, the data of vapor pressures : B : 754.12, T : 289.71, X : 91.19. Unit is mm Hg. The pressure P is 0.5 atm. The value of k for each substance is k = (vapor pressure) / P. (a) Calculate k for B, T and X. Let L / V = 0.65. (b) By using the equation V = F / [ (L / V) + 1 ], find the value of V when F = 100, then what is the value of L?

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