what is the Function of beta-mercaptoethanol in cell
culture medium ?
- 2 Answers
- 43293 Views
- Invitrogen, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / francois niyonsaba
To my knowledge, it is said that 2-ME is important for
lymphocyte or other cell cultures because the amino acid
cysteine is in short supply in FBS used in culture media,
while there is plenty of cystine. For example, T cells are
unable to transport cystine into the cytosol, where it must
be converted into cysteine. 2-ME reduces cystine into a
form that can be transported by the cells and is then
converted to cysteine needed for cell growth.
Another reason is that 2-ME, as a reducing agent, can break
down many of the toxic metabolites produced by cells in
culture thus improving the environment around the cells.
Francois NIYONSABA, M.D., Ph.D.
Associate Professor
Atopy/Allergy Research Center
Juntendo University School of Medicine
2-1-1 Hongo, Bunkyo-Ku
Tokyo 113-8421 JAPAN
Tel: +81-3-5802-1591
Fax: +81-3-3813-5512
E-mail: francois@juntendo.ac.jp
| Is This Answer Correct ? | 78 Yes | 5 No |
Answer / chanchai
Beta-mercaptoethanol in cell culture medium was used to
differentiate from peripheral blood into neuronal cells
| Is This Answer Correct ? | 4 Yes | 39 No |
Will Macrophage Survive without changing media ?
Explain the modes of Natritions of plants ?
Is it possible for the transfection of primary neuronal cell cultures?
DMSO or Ethanol which is relatively less toxic to cells?
what is Co-transfection ?
Where in the cell can ribosomes be found?
How is the covering membrane of the vacuoles called?
Give an account of Isolation of outer membrane of protein?
how to Stimulate the THP-1 into monocyte?
What is the way followed by the energy absorbed by plants to be used in photosynthesis?
How to gate Apoptosis?
What is the name of the inner surface of the myocardium that is lined with a layer of endothelial cells?
Micro Biology 
