MICROBIOLOGICAL ENGINEERING - QUESTION 28.2 : A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells occupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).

1 Answers  

---

BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.2 : (a) In order to determine the slurry volume (L) in chromatography, arrange the formula using the following parameters : slurry concentration (%), slurry volume (L), settled chromatography medium volume (L), 100. (b) In order to determine the chromatography medium volume (L) in gel filtration chromatography, arrange the formula using the following parameters : sample volume to be processed (L), chromatography medium volume (L), sample volume in percent of total column volume (%), 100.

1 Answers  

---

Question 70 - According to Adolf Eugen Fick (1829 - 1901) : rate of diffusion v increases with less wall thickness t, increased area A and decreased molecular weight of a fluid M. The diffusion constant D decreased with increasing M. (a) By assuming v, t, dP, A, M and D changes proportionally of each other, find the equation of v as a function of t, dP, A and D. (b) The ratio of self diffusion constant D, at T = 273 K and P = 0.1 MPa, for gases B and C are 1.604 : 0.155. If only 2 gases exist in such a system : hydrogen and nitrogen, find the type of gas for B and C with reference to their molecular weights M. (c) By using the equation of kinetic energy 0.5 MV = constant where V = square of v, find the ratio of V for B and V for C, or V(B) / V(C), as a function of M(B) and M(C), where M(B) is molecular weight of B and M(C) the molecular weight of C : Graham's Law of Diffusion.

1 Answers  

---

Mention some of the specialized grinding methods?

0 Answers  

---

why PH scale range is 14? why it is not less or not greater than 14? solution of HCl or hydrogen containing liquid has a PH. but if the solution is KMnO4 here not hydrogen power does it PH? explain plz....

1 Answers  

---

y we do not differ b/w atomic mass and atomic wieght? from engrtauseef2008@gmail.com

0 Answers  

---

What is a good source of equations for calculating discharge flowrates from accidental releases?

0 Answers  

---

QUANTUM CHEMISTRY AND CHEMICAL ENGINEERING - EXAMPLE 31.5 : In a wavefunction, let P(x) = A cos kx + B sin kx. By using the boundary conditions of x = 0 and x = l, where P(0) = P(l) = 0, prove by mathematical calculation that P(x) = B sin (npx / l) where p = 22 / 7 approximately, n is a rounded number. A, B and k are constants.

1 Answers  

---

why screw compressor use for ammonia compression?

0 Answers   Deepak Fertilisers,

---

If flow rate of natural gas mixture in sweating gas unite is 460000 m3/day Can it be converted into mole/day?

1 Answers   Shell,

---

How to estimate the efficiency of a pump?

2 Answers   OGDCL,

---

ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.23 : An engineering company would like to produce 10000 units of control instruments. Let selling price per unit = $10, variable cost per unit = $2, overall fixed cost = $30000. (a) Calculate the income obtained when all units are sold out successfully. (b) Find the overall cost of production. (c) Calculate the percentage of gross profit obtained based on the answers in (a) and (b). (d) Find the minimum units that need to be sold out successfully in order to prevent losses. (e) How many minimum units of instruments that need to be produced in order to prevent losses, if all units produced are sold out successfully?

1 Answers  

---