Question 89 - A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells ocupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).

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In DM plant, If pH in anion outlet is 7.2 and Mixed bed pH is 6.2, wat could be the reason.

8 Answers   Cipla, Jindal,

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Given, T1=600C, constant temperature of wall 800 , length of wit exchanger L=3m, dia=25 mm, Cp=14.91 KJ/kg.K, density ρ=914 kg/m3,mean velocity vm= 2 m/s, film heat transfer coefficient hf=72 Kcal/h.m2.K, what is the outlet temperature?

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DEAR SIR, IS IT ANY BARC TECHNICAL OR INTERVIEW QUESTION BOOK IN CHEMICAL ENGG LAST YEARS IN MARKET? ALSO GIVE THE PATTERN HOW TO PREPARE AND WHAT TYPE OF QUESTION THEY ASKED?

0 Answers   BARC, Reliance,

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which type of packing gives large interfacial area stacked or random?

2 Answers   NPCIL,

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What does the catalytic converter on an automobile do?

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Explain what are the disadvantages of using gear pumps?

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What is a good method of analyzing powders for composition?

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POLYMER ENGINEERING - QUESTION 24.2 : Let C% be the fractional crystallinity, Rs = density of sample, Ra = density of amorphous form and Rc = density of crystalline form. In a polymer, these unknowns could be related by the equation C% = (Rc / Rs) (Rs - Ra) / (Rc - Ra). (a) Find the equation of Rc as a function of C%, Rs and Ra. (b) Two samples of a polymer, C and D exist. For sample C, C% = 0.513 when Rs = 2.215 unit. For sample D, C% = 0.742 when Rs = 2.144 unit. Both samples C and D have the same values of Ra and Rc. Find the values of Ra and Rc in 6 decimal places.

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Heat transfer: In a triple effect evaporator, the heat transfer for an evaporator is calculated as q = UA (TI - TF) where TI is the initial temperature, TF is the final temperature; U and A are constants. Given that heat transfer for the first evaporator : q(1) = UA (TI - TB); second evaporator : q(2) = UA (TB - TC); third evaporator : q(3) = UA (TC - TF) where q(x) is the heat transfer function, TB is the temperature of second inlet and TC is the temperature of third inlet, prove that the overall heat transfer Q = q(1) q(2) q(3) = UA (TI - TF).

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What is a good way to get started in doing a plant-wide steam consumption analysis?

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Are there any methods of preventing cracking of carbon steel welds in refining environments?

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