Question 94 - The resolution of separation, Rs for chromatography is given by the formula Rs = (difference in retention time) / (average width at the base). In a chromatogram, 3 peaks a, b and c are found. Average widths W at the bases of the solutes are : Wa = 20 s, Wb = 40 s, Wc = 30 s. Resolutions of separation, Rs for solutes b and c in comparison to a are 2 and 4 respectively. The differences in retention times T for b and c in comparison to a are (Tb - Ta) and (Tc - Ta), Ta = Tc - Tb : (a) Form 2 equations involving Rs as a function of Wa, Wb, Wc, Ta, Tb and Tc. (b) Find the values of Ta, Tb and Tc.
- 1 Answers
- 1790 Views
- I also Faced
- E-Mail Answers
Answer 94 - Reference formula Rs = (difference in retention time) / (average width at the base) is used. (a) First equation : Rs = (Tb - Ta) / [ (Wa + Wb) / 2 ] = 2 (Tb - Ta) / (Wa + Wb). Second equation : Rs = (Tc - Ta) / [ (Wa + Wc) / 2 ] = 2 (Tc - Ta) / (Wa + Wc). (b) Substitute Ta = Tc - Tb, Wa = 20 s, Wb = 40 s and Wc = 30 s into first equation and second equation. First equation : Rs = 2 = 2 [ Tb - (Tc - Tb) ] / (20 + 40) = (2 Tb - Tc) / 30, 2 Tb - Tc = 60. Second equation : Rs = 4 = 2 [ Tc - (Tc - Tb) ] / (20 + 30) = Tb / 25, Tb = 100 s. Substitute Tb = 100 s into first equation gives 2 Tb - Tc = 2 x 100 - Tc = 200 - Tc = 60, then Tc = 200 - 60 = 140 s. Then Ta = Tc - Tb = 140 - 100 = 40 s. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
| Is This Answer Correct ? | 0 Yes | 0 No |
What are the different characteristics that can affect the flow of bulk solids and how?
Explain how can you estimate a gas flow based on two pressure measurements?
What are some common causes of gas pipeline vibration 20 carbon steel line?
What is the procedure to estimate the friction factor involved in heat exchanger tubes?
What are the some common problems associated with dense phase pneumatic conveying?
How can one determine the particle size distribution for a given bulk solid?
testing formula for oxygen by ammonium chloride,ammonium hydroxide and copper
What is higher heating value and lower heating value? Is it positive or negative?
when selecting psv(pressure safety valve),what are all things we have to keep it in mind?
A concentric, counter-current heat exchanger is used to cool lubricating oil. Water is used as the coolant. The mass flow rate of oil into the heat exchanger is 0.1 kg / s = FO. For oil, the inlet temperature TIO = 100 degree Celsius and the outlet temperature TOO = 55 degree Celsius. For water, the inlet temperature TIW = 35 degree Celsius and the outlet temperature TOW = 42 degree Celsius. What is the mass flow rate of water in kg / s, FW needed to maintain these operating conditions? Constant for heat capacity of oil is CO = 2131 J /(kg K) and for water is CW = 4178 J /(kg K). Use the equation (FO)(CO)(TIO – TOO) = (FW)(CW)(TOW – TIW).
CHEMICAL MATERIAL BALANCE - EXAMPLE 2.1 : Two methanol-water mixtures are contained in separate tanks. The first mixture contains 40.0 wt % methanol and the second contains 70.0 wt % methanol. If 200 kg of the first mixture is combined with 150 kg of the second, what are the mass and composition of the product? The symbol of weight percent is wt %.
hi.i m student of chemical engg. and studying in 5 th sem.how can i improve my technical knowledge..can u sugest me a link like book or any website..?
-
Civil Engineering (5085) - Mechanical Engineering (4451)
- Electrical Engineering (16632)
- Electronics Communications (3918)
- Chemical Engineering (1095)
- Aeronautical Engineering (239)
- Bio Engineering (96)
- Metallurgy (361)
- Industrial Engineering (259)
- Instrumentation (3014)
- Automobile Engineering (332)
- Mechatronics Engineering (97)
- Marine Engineering (124)
- Power Plant Engineering (172)
- Textile Engineering (575)
- Production Engineering (25)
- Satellite Systems Engineering (106)
- Engineering AllOther (1379)
