Dear Sir,
This is Thirunavukkarasu am working in Ordain Health Care
AR&D, Am Having one problem While doing method development
my question Metadoxine and Pyridoxine same chemical nature
and structural activity i tried lot of methods but Analyte
eluted same Retantion time i cant separate both peaks.
solubility, pH both are same only only difference melting
point metadoxine 210 C Pyridoxine 102 C please do the help
needful for me
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Answer Posted / bpatel1773
Dear ,
I thing you want to isolate both compound , You can try
derivatization method using bezoyl chloride or densyl
chloride . Bezoyl chloride will react with only metadoxine
and it will eluted late on HPLC . This way must work .
Benzoy chloride will react at RT only
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